TY  - JOUR
PY  - 2001//
TI  - Interaction between duodenase and alpha1-proteinase inhibitor
JO  - Biochemistry (Moscow)
A1  - Gladysheva, I. P.
A1  - Popykina, N. A.
A1  - Zamolodchikova, T. S.
A1  - Larionova, N. I.
SP  - 682
EP  - 687
VL  - 66
IS  - 6
N2  - The interaction between duodenase, a newly recognized serine proteinase belonging to the small group of Janus-faced proteinases, and alpha1-proteinase inhibitor (alpha1-PI) from human serum was investigated. The stoichiometry of the inhibition was 1.2 mol/mol. The presence of a stable enzyme-inhibitor complex was shown by SDS-PAGE. The mechanism of interaction between duodenase and alpha1-PI was shown to be of the suicide type. The equilibrium and inhibition constants are 13 +/- 3 nM and (1.9 +/- 0.3).105 M-1.sec-1, respectively. Based on the association rate constant of the enzyme-inhibitor complex and localization of duodenase and alpha1-PI in identical compartments, alpha1-PI is suggested to be a duodenase inhibitor in vivo.<p /> <p>Language: en</p>
LA  - en
SN  - 0006-2979
UR  - http://dx.doi.org/10.1023/a:1010267500296
ID  - ref1
ER  -