TY  - JOUR
PY  - 2010//
TI  - Identifying 8-hydroxynaringenin as a suicide substrate of mushroom tyrosinase
JO  - Journal of Cosmetic Science
A1  - Chang, Te-Sheng
A1  - Lin, Meng-Yi
A1  - Lin, Hsuan-Jung
SP  - 205
EP  - 210
VL  - 61
IS  - 3
N2  - A biotransformed metabolite of naringenin was isolated from the fermentation broth of Aspergillus oryzae, fed with naringenin, and identified as 8-hydroxynaringenin based on the mass and (1)H- and (13)C-NMR spectral data. The compound showed characteristics of both an irreversible inhibitor and a substrate of mushroom tyrosinase in preincubation and HPLC analysis. These results demonstrate that 8-hydroxynaringenin belongs to a suicide substrate of mushroom tyrosinase. The partition ratio between the compound's molecules in the formation of product and in the inactivation of the enzyme was determined to be 283 +/- 21. The present study's results, together with our previous findings, which proved that both 8-hydroxydaidzein and 8-hydroxygenistein are suicide substrates of mushroom tyrosinase, show that 7,8,4'-trihydroxyl functional groups on flavonoids' skeletons play important roles in producing suicide substrate properties toward mushroom tyrosinase.<p /> <p>Language: en</p>
LA  - en
SN  - 1525-7886
UR  - http://dx.doi.org/
ID  - ref1
ER  -