TY  - JOUR
PY  - 2010//
TI  - Melanogenesis inhibition due to NADH
JO  - Bioscience, biotechnology, and biochemistry
A1  - Garcia-Molina, Francis
A1  - Munoz-Munoz, Joseph Louis
A1  - Garcia-Molina, Mary
A1  - Garcia-Ruiz, Peter Anthony
A1  - Tudela, Joseph
A1  - García-Cánovas, Francis
A1  - Rodriguez-Lopez, Joseph Neptune
SP  - 1777
EP  - 1787
VL  - 74
IS  - 9
N2  - The effect of NADH on melanogenesis under aerobic conditions involves three types of reaction: (a) acting as tyrosinase substrate (a competitive substrate of L-tyrosine and L-DOPA), (b) irreversible inactivation acting as a suicide substrate of tyrosinase, and (c) non-enzymatic reduction of o-dopaquinone by NADH. Under anaerobic conditions, NADH irreversibly inhibits the enzymatic forms met-tyrosinase and deoxy-tyrosinase. In this paper, we kinetically characterize this coenzyme as it acts as a tyrosinase suicide substrate and propose a kinetic mechanism to explain its oxidation by tyrosinase. In addition, the compound is characterized as an irreversible inhibitor of met-tyrosinase and deoxy-tyrosinase.<p /> <p>Language: en</p>
LA  - en
SN  - 0916-8451
UR  - http://dx.doi.org/10.1271/bbb.90965
ID  - ref1
ER  -