Citation

Harper JD, Martel PA, O'Donnell CM. J. Anal. Toxicol. 1989; 13(1): 31-36.

Affiliation

Toxi-Lab, Inc., Irvine, California 92718.

Copyright

(Copyright © 1989, Preston Publications)

DOI

unavailable

PMID

2709825

Abstract

An analytical scheme composed of one normal-phase thin-layer chromatographic (TLC) method, one reversed-phase thin-layer chromatographic (RPTLC) method, and sequential analyte detection through four stages of color reactions is described. Eighty-one basic or neutral drugs were analyzed with this scheme and seventy-four were uniquely characterized with 95% confidence. Six of the remaining seven formed three unresolved pairs. The scheme was evaluated by mean list length analysis and shown to offer analyte resolution similar to that of a scheme of TLC and gas chromatography (GC) with nonspecific detection. Nine out of ten unknowns from the field of eighty-one drugs were uniquely identified with 95% confidence by the TLC/RPTLC scheme. The tenth unknown was not completely resolved from its isomer, but was statistically the more probable candidate.

Language: en