Citation

Astier A, Baud FJ. J. Chromatogr. B Biomed. Appl. 1995; 667(1): 129-135.

Affiliation

Laboratoire de Toxicologie, CHU Henri Mondor, Créteil, France.

Copyright

(Copyright © 1995, Elsevier Publishing)

DOI

unavailable

PMID

7663675

Abstract

Hydroxocobalamin (OHCbl) is a powerful antidote for cyanide poisoning, via the formation of non-toxic cyanocobalamin (CNCbl). Plasmatic cobalamins were measured at 361 nm, after enrichment and purification on a short C18 precolumn (1% acetic acid; 1 ml min-1; 2 min), by back-flush elution on a C18 ODS-2 column [0.1 M sodium dihydrogenphosphate-methanol (63:27, v/v) (pH 4.0); 0.80 ml min-1]. The precision was 3.21 and 3.54% for 10 microM OHCbl and CNCbl, respectively. The method was used to study the pharmacokinetics of OHCbl and the formed CNCbl in severely poisoned patients.

Language: en