Citation

Polettini A. J. Chromatogr. B Biomed. Sci. Appl. 1999; 733(1-2): 47-63.

Affiliation

Department of Legal Medicine and Public Health, University of Pavia, Italy. apole@unipv.it

Copyright

(Copyright © 1999, Elsevier Publishing)

DOI

unavailable

PMID

10572974

Abstract

The introduction of hyphenated chromatographic-spectroscopic techniques represented a substantial step-forward for Systematic Toxicological Analysis (STA), increasing the amount and quality of information obtainable from the analysis of a biological sample, and enhancing the possibilities of identifying unknown drugs and poisons. STA methods based either on GC-MS or on HPLC-UV published in the last decade are reviewed in this paper. The different analytical phases, i.e. sample preparation (pretreatment, extraction, derivatisation), chromatographic separation and detection/identification are examined in detail in order to emphasise the complementarity of the two approaches. In addition, the first STA method based on HPLC-MS is illustrated and some applications of TLC-UV to drug screening are also described. Finally, an overview of semi- and fully-automated STA methods is given.

Language: en