Citation

Oukkache N, Chgoury F, Lalaoui M, Cano AA, Ghalim N. J. Venom Anim. Toxins Incl. Trop. Dis. 2013; 19(1): 5.

Affiliation

Laboratory of Venoms and Toxins, Pasteur Institute of Morocco, 1 Place Louis Pasteur, Casablanca 20360, Morocco. nghalim@yahoo.fr.

Copyright

(Copyright © 2013, Universidade Estadual Paulista. Centro de Estudos de Venenos e Animais Peçonhentos)

DOI

10.1186/1678-9199-19-5

PMID

23849043

Abstract

BACKGROUND: The present study compared two methods used successfully in a large-scale program for the collection of scorpion venoms, namely the milking of adult scorpions via manual and electrical stimulation. RESULTS: Our immunobiochemical characterizations clearly demonstrate that regularly applied electrical stimulation obtains scorpion venom more easily and, most importantly, in greater quantity. Qualitatively, the electrically collected venom showed lack of hemolymph contaminants such as hemocyanin. In contrast, manual obtainment of venom subjects scorpions to maximal trauma, leading to hemocyanin secretion. Our study highlighted the importance of reducing scorpion trauma during venom milking. CONCLUSIONS: In conclusion, to produce high quality antivenom with specific antibodies, it is necessary to collect venom by the gentler electrical stimulation method.

Language: en