Citation

Li R, Yu H, Xue W, yue Y, Liu S, Xing R, Li P. J. Proteomics 2014; 106: 17-29.

Affiliation

Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China. Electronic address: pcli@qdio.ac.cn.

Copyright

(Copyright © 2014, Elsevier Publishing)

DOI

10.1016/j.jprot.2014.04.011

PMID

24747124

Abstract

Jellyfish Stomolophus meleagris is a very dangerous animal because of its strong toxicity. However, the composition of the venom is still unclear. Both proteomics and transcriptomics approaches were applied in present study to investigate the major components and their possible relationships to the sting. The proteomics of the venom from S. meleagris was conducted by tryptic digestion of the crude venom followed by RP-HPLC separation and MS/MS analysis of the tryptic peptides. The venom gland transcriptome was analyzed using a high-throughput Illumina sequencing platform HiSeq™ 2000 with de novo assembly. A total of 218 toxins were identified including C-type lectin, phospholipase A2 (PLA2), potassium channel inhibitor, protease inhibitor, metalloprotease, hemolysin and other toxins, most of which should be responsible for the sting. Among them, serine protease inhibitor, PLA2, potassium channel inhibitor and metalloprotease are predominant, representing 28.44%, 21.56%, 16.06% and 15.14% of the identified venom proteins, respectively. Overall, our combined proteomics and transcriptomics approach provides a systematic overview of the toxins in the venom of jellyfish S. meleagris and it will be significant to understand the mechanism of the sting BIOLOGICAL SIGNIFICANCE: Jellyfish Stomolophus meleagris is a very dangerous animal because of its strong toxicity. It often bloomed in the coast of China in recent years and caused thousands of people stung and even deaths every year. However, the components caused to sting is still unknown yet. In addition, no study about the venomics of jellyfish Stomolophus meleagris has been reported. In present study, both proteomics and transcriptomics approaches were applied to investigate the major components related to the sting. The result showed that major component included C-type lectin, phospholipase A2, potassium channel inhibitor, protease inhibitor, metalloprotease, hemolysin and other toxins, which should be responsible for the effect of sting. This is first research about the venomics of jellyfish Stomolophus meleagris. It will be significant to understand the mechanism of the biological effects and helpful to develop ways to deal with the sting.

Language: en