Citation

Franco de Oliveira SCWSE, Zucoloto AD, de Oliveira CDR, Hernandez EMM, Fruchtengarten LVG, de Oliveira TF, Yonamine M. J. Mass Spectrom. 2019; 54(7): 600-611.

Affiliation

School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil.

Copyright

(Copyright © 2019, John Wiley and Sons)

DOI

10.1002/jms.4369

PMID

31066158

Abstract

A fast and simple approach to overcome challenges in emergency toxicological analysis, using UHPLC-MS/MS has been developed, for the detection of analytes in blood and urine samples from the following drug classes: analgesics, benzodiazepines, antidepressants, anticonvulsants, drugs of abuse and pesticides. These substances are relevant in the context of emergency toxicology in Brazil. The sample preparation procedure was relatively easy and fast to perform. The method was fully validated giving limits of in the range of 0.5 ng mL^-1 and 20 ng mL^-1 for blood and urine samples. The intra and inter day precision and accuracy were considered adequate for all analytes once the RSD (%) were lower than 20% for QC low and lower than 15% for CQ medium and high. The developed method was successfully applied to 320 real samples collected at the Poison Control Center of São Paulo and 89.1% have shown to be positive for some of the analytes. This confirms its applicability and importance to emergency toxicological analysis and it could be very useful in both fields of clinical and forensic toxicology.

This article is protected by copyright. All rights reserved.

Language: en

Keywords

Analytical toxicology; Emergency; Multi-analyte; UHPLC-MS/MS