Citation

Concheiro M, de Castro A, Quintela O, López-Rivadulla M, Cruz A. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2006; 832(1): 81-89.

Affiliation

Forensic Toxicology Service, Institute of Legal Medicine, University of Santiago de Compostela, Spain, C/San Francisco s/n, 15782 Santiago de Compostela, Spain.

Copyright

(Copyright © 2006, Elsevier Publishing)

DOI

10.1016/j.jchromb.2005.12.047

PMID

16436334

Abstract

A method, using 0.2 ml of plasma, was designed for the simultaneous determination of morphine, 6-monoacetylmorphine, amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB, benzoylecgonine and cocaine. The drugs were analysed by LC-MS, after solid phase extraction in the presence of the deuterated analogues. Reversed phase separation on an Atlantis dC18 column was achieved in 10 min, under gradient conditions. The method was full validated, including linearity (2-250 ng/ml, r2>0.99), recovery (>50%), within-day and between-day precision and accuracy (CV and bias <15%), limit of detection (0.5 and 1 ng/ml) and quantitation (2 ng/ml), relative ion intensities and no matrix effect was observed. The procedure showed to be sensitive and specific, and was applied to 156 real cases from road fatalities (7.1% cases positive to cocaine and 0.6% to designer drugs).

Language: en