Citation

Zhou YC, Wang B, Cai J, Xu YZ, Qin XS, Ha S, Cong B, Chen JH, Deng JQ. J. Vis. Exp. 2023; 2023(201).

Copyright

(Copyright © 2023, MYJoVE Corporation)

DOI

10.3791/65792

PMID

unavailable

Abstract

Diatom testing is an essential auxiliary means in forensic practice to determine whether the corpse drowned in water and to infer the drowning location. Diatom testing is also an important research content in the field of the environment and plankton. The diatom molecular biology testing technology, which focuses on diatom DNA as the primary research object, is a new method of diatom testing. Diatom DNA extraction is the basis of diatom molecular testing. At present, the kits commonly used for diatom DNA extraction are expensive, which increases the cost of carrying out related research. Our laboratory improved the general whole blood genomic DNA rapid extraction kit and obtained a satisfactory diatom DNA extraction effect, thus providing an alternative economical and affordable DNA extraction solution based on glass beads for related research. The diatom DNA extracted using this protocol could satisfy many downstream applications, such as PCR and sequencing.

Language: en