Citation

Goncerz G, Tomaszewski KA, Pasternak A, Głowacki R, Wróbel A, Rokita E, Podolec P. J. Heart Valve Dis. 2014; 23(5): 545-549.

Copyright

(Copyright © 2014, ICR Publishers)

DOI

unavailable

PMID

unavailable

Abstract

BACKGROUND AND AIM OF THE STUDY: The study aim was to develop an in-vitro model of human aortic valve homograft (AVH) mineralization, as a basis for further research on the subject of calcific aortic valve disease (CAVD).; METHODS: Human aortic valves were excised during autopsy (12-24 h post-mortem) from donors who had died due to accident or suicide. The mean (±SD) donor age was 32.5 ± 11.4 years. Under sterile conditions each aortic cusp (three cusps per valve) was cut in half to provide a total of 240 tissue samples. AVH mineralization was assessed in solutions with different Ca and P concentrations and CaxPO4 ionic product values. The impact of time on aortic valve mineralization rate was assessed using energy-dispersive X-ray fluorescence (ED-XRF).; RESULTS: The model showed that human AVH mineralization can be best observed in a solution with a CaxPO4 ionic product of 2.2 mmol2/l2. The optimum incubation time for observing AVH mineralization was 21 days.; CONCLUSION: A novel in-vitro AVH mineralization model was developed for use in future studies.

Language: en

Keywords

Humans; human; pathology; phosphorus; Phosphorus; calcium; Calcium; chemistry; aorta valve; Aortic Valve; aorta valve stenosis; Aortic Valve Stenosis; Spectrometry, X-Ray Emission; allograft; Allografts; calcinosis; Calcinosis; calcium phosphate; Calcium Phosphates; Culture Media; culture medium; spectrometry