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Citation |
Mireles R, Ramirez-Ramirez J, Alcalde M, Ayala M. J. Fungi (Basel) 2021; 7(8): e608. |
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Copyright |
(Copyright © 2021, MDPI: Multidisciplinary Digital Publications Institute) |
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DOI |
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PMID |
34436147 |
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PMCID |
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Abstract |
Ethers can be found in the environment as structural, active or even pollutant molecules, although their degradation is not efficient under environmental conditions. Fungal unspecific heme-peroxygenases (UPO were reported to degrade low-molecular-weight ethers through an H2O2-dependent oxidative cleavage mechanism. Here, we report the oxidation of a series of structurally related aromatic ethers, catalyzed by a laboratory-evolved UPO (PaDa-I) aimed at elucidating the factors influencing this unusual biochemical reaction. Although some of the studied ethers were substrates of the enzyme, they were not efficiently transformed and, as a consequence, secondary reactions (such as the dismutation of H2O2 through catalase-like activity and suicide enzyme inactivation) became significant, affecting the oxidation efficiency. The set of reactions that compete during UPO-catalyzed ether oxidation were identified and quantified, in order to find favorable conditions that promote ether oxidation over the secondary reactions. Language: en |
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Keywords |
biodegradation; ether oxidation; fungal peroxygenase; suicide inactivation; xenobiotic transformation |