Citation

Kage S, Nagata T, Kudo K. J. Chromatogr. 1991; 564(1): 163-169.

Copyright

(Copyright © 1991, Elsevier Publishing)

DOI

10.1016/0378-4347(91)80078-q

PMID

1860910

Abstract

A sensitive and simple method to determine polysulphides in human blood, using an extractive alkylation technique and gas chromatography, has been devised. Polysulphides were alkylated with pentafluorobenzyl bromide, and then converted into bis(pentafluorobenzyl)disulphide by desulphuration with potassium cyanide. The disulphide was analysed qualitatively by mass fragmentography and quantitatively by gas chromatography with electron-capture detection. The lower limit of detection was 0.005 mumol/ml. Field testing in a suicide case confirmed the validity of the method.

Language: en

Keywords

Alkylation; Chromatography, Gas; Fluorobenzenes; Gas Chromatography-Mass Spectrometry; Humans; Potassium Cyanide; Sulfides