Citation

Usui K, Hishinuma T, Yamaguchi H, Tachiiri N, Goto J. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2006; 831(1-2): 105-109.

Copyright

(Copyright © 2006, Elsevier Publishing)

DOI

10.1016/j.jchromb.2005.11.051

PMID

16380301

Abstract

Rapid and reliable methods for identification of chlorhexidine (CHD) and nonylphenolethoxylates (NPEOn) in antiseptic and hemolyzed blood using electrospray ionization mass spectrometry (ESI-MS) were developed. Fragmental analysis provides accurate evidence for the presence of CHD in the samples. For the determination of CHD in hemolyzed blood, the method was also developed using LC-ESI-MS. Linearity of calibration curve was obtained over the concentration range of 0.1-11 microg/mL with residuals from -4.3 to 6.7%. We applied the methods to the case of suicidal injection of antiseptic and successfully detected CHD and NPEOn from hemolyzed blood. The CHD concentration was 352 microg/mL.

Language: en

Keywords

Adult; Anti-Infective Agents, Local; Chlorhexidine; Chromatography, High Pressure Liquid; Ethylene Glycols; Female; Hemolysis; Humans; Infusions, Intravenous; Spectrometry, Mass, Electrospray Ionization; Suicide; Surface-Active Agents