Citation

Alexeev D, Baxter RL, Campopiano DJ, Kerbarh O, Sawyer L, Tomczyk N, Watt R, Webster SP. Org. Biomol. Chem. 2006; 4(7): 1209-1212.

Copyright

(Copyright © 2006, Royal Society of Chemistry)

DOI

10.1039/b517922j

PMID

16557306

Abstract

The irreversible inhibition of 8-amino-7-oxononanoate synthase by trifluoroalanine involves decarboxylative defluorination of the inhibitor-PLP aldimine followed by attack of the conjugated imine by the amino group of the active site lysine to afford a covalently bound difluorinated intermediate which can subsequently undergo further HF losses and hydrolysis to afford a 2-(pyridoximine phosphate) acetoyl protein adduct.

Language: en

Keywords

Acyltransferases; Alanine; Binding Sites; Catalysis; Hydrolysis; Ligases; Lysine; Models, Molecular; Protein Conformation