Citation

Munoz-Munoz JL, García-Molina F, Arribas E, García-Ruiz PA, Tudela J, García-Cánovas F, Rodriguez-Lopez JN. J. Enzyme Inhib. Med. Chem. 2011; 26(5): 728-733.

Copyright

(Copyright © 2011, Informa - Taylor and Francis Group)

DOI

10.3109/14756366.2010.548811

PMID

21299451

Abstract

Tetrahydrobiopterin (BH(4)), methyl-tetrahydropterin (MBH(4)) and dimethyl-tetrahydropterin (DMBH(4)) are oxidized by tyrosinase in a process during which the suicide inactivation of tyrosinase may occur. From the kinetic study of this process, [Formula: see text] (apparent maximum constant for the suicide inactivation), [Formula: see text] (Michaelis constant for the substrate) and r (number of turnovers that the enzyme makes before the inactivation) can be obtained. From the results obtained, it can be deduced that the velocity of the inactivation governed by ([Formula: see text]) and the potency of the same ([Formula: see text]) follow the order: BH(4) > MBH(4) > DMBH(4).

Language: en

Keywords

Kinetics; Molecular Structure; Enzyme Inhibitors; Enzyme Activation; Monophenol Monooxygenase; Pterins