Citation

Wietecha-Posłuszny R, Lech T, Kościelniak P. J. Forensic Sci. 2011; 56(2): 518-521.

Affiliation

Laboratory for Forensic Chemistry, Faculty of Chemistry, Jagiellonian University, 3 Ingardena St., 30-060 Kraków, Poland. Institute of Forensic Research, 9 Westerplatte St., 31-033 Kraków, Poland.

Copyright

(Copyright © 2011, American Society for Testing and Materials, Publisher John Wiley and Sons)

DOI

10.1111/j.1556-4029.2010.01660.x

PMID

21342197

Abstract

Three spectrometric methods, that is, spectrofluorimetry (SF), atomic absorption spectrometry with electrothermal atomization (ET-AAS), and atomic fluorescence spectrometry with hydride generation (HG-AFS) were used for the determination of total selenium in biological samples taken from postmortem material in a case of acute selenium compound poisoning. The precision of the SF, ET-AAS, and HG-AFS methods (RSD, n = 10) was found to be in the ranges of 10.0-15.0, 3.0-6.0 and 1.0-1.5%, respectively, and the detection limit was 10.0, 4.0, and 0.1 μg/L of Se, respectively. In the case of HG-AFS, the analytical procedure takes less time and is less laborious than the other methods considered. The obtained results show the usefulness of the HG-AFS method as a supplementary analytical tool to the SF and ET-AAS methods with respect to the determination of selenium as well as the possibility of using this method as a primary one in forensic toxicology practice.

Language: en