First approach of a methodological set‐up for selenomethionine chiral speciation in breast and formula milk using high‐performance liquid chromatography coupled to atomic fluorescence spectroscopy

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First approach of a methodological set‐up for selenomethionine chiral speciation in breast and formula milk using high‐performance liquid chromatography coupled to atomic fluorescence spectroscopy
Citation	Gómez‐Ariza JL, Bernal‐Daza V, Villegas‐Portero MJ. Appl. Organomet. Chem. 2007; 21(6): 434-440.
Copyright	(Copyright © 2007, John Wiley and Sons)
DOI	10.1002/aoc.1239
PMID	unavailable
Abstract	The chiral speciation of selenomethionine in breast and formula milk based on species separation by high-performance liquid chromatography followed by online microwave-assisted digestion and detection with hydride generation atomic fluorescence spectrometry (HPLC-MAD-HG-AFS) requires severe sample manipulation to avoid matrix influence. Sample clean-up for fat and protein elimination using centrifugation and ultrafiltration was optimized, and selenomethionine preconcentration based on cation exchange solid-phase extraction was studied and optimized. The resulting procedure is suitable for chiral selenium speciation in infant milk with detection limits of 3.1 and 3.5 ng ml−1 as Se for L-selenomethionine and D-selenomethionine, respectively. The time necessary for the analysis, about 90 min, including sample clean-up, analyte preconcentration and chromatographic separation, makes the approach suitable for routine analysis. Copyright © 2007 John Wiley & Sons, Ltd.