Citation

Sibille I, Duverneuil C, Lorin de la Grandmaison G, Guerrouache K, Teissiere F, Durigon M, de Mazancourt P. Forensic Sci. Int. 2002; 125(2-3): 212-216.

Affiliation

Department of Pathology and Forensic Medicine, Raymond Poincaré Hospital, 92380 Garches, France.

Copyright

(Copyright © 2002, Elsevier Publishing)

DOI

unavailable

PMID

11909666

Abstract

Identification of spermatozoa is the biological evidence most often sought in specimens from rape victims. Absence of spermatozoa usually terminates biological investigations, and the victim's testimony can be contested. We assessed the utility and reliability of PCR amplification using Y-chromosomal STR polymorphisms in specimens from female victims of sexual assault with negative cytology. One hundred and four swabs without spermatozoa detected by cytology were collected from 79 alleged sexually assaulted female victims and amplification of Y-STR and of amelogenin was performed.Overall, Y-chromosome was detected and evidenced sexual penetration in 28.8% of swabs. In the population of victims examined more than 48 h after the sexual assault, Y-STR were still evidenced in 30% of the cases. These results show that swabs should be taken from victims for Y-chromosome DNA typing even after long delays between sexual assault and medical examination.

Language: en