Citation

Moaleş D, Spac AF, Gavriliu C, Bădără M, Iftimie D, Butnaru E. Rev. Med. Chir. Soc. Med. Nat. Iasi 2011; 115(4): 1283-1287.

Vernacular Title

Determinarea concentratiei metanolului prin GC-MS intr-un caz de intoxicatie

Affiliation

Disciplina Toxicologie, Facultatea de Farmacie, Universitatea de Medicină şi Farmacie "Gr. T. Popa" Iaşi.

Copyright

(Copyright © 2011, Societatii de Medici si Naturalisti)

DOI

unavailable

PMID

22276484

Abstract

Due to the consumption of alcoholic beverages with methanol content above the admitted limit, a woman from Bacău died at the Bacău County Hospital after 9 hours of hospitalization. MATERIALS AND METHODS: For the toxicological examination blood, liver, bile, stomach, intestine and kidney samples were analyzed in order to establish the concentration of methanol. Determination of methanol was carried out by gas chromatography coupled with mass spectrometry (GC-MS), analyzing the distillates obtained from blood and organs. We used an Agilent Technologies 7890 A gas chromatograph coupled to an Agilent Technologies 7683 C spectrometer. Helium was employed as mobile phase and a Phenomenex Zebron ZW-Plus polar column represented the stationary phase. Spectra acquisition mode: SIM, mass range followed: 15-500 atomic mass units, interpretation of the obtained results: Agilent Technologies ChemStation software. RESULTS: After peak integration on the chromatograms, the following values of methanol concentration were obtained: 1.720 g per thousand in blood, 0.795 g per thousand in liver, 2.661 g per thousand in bile, 2.287 g per thousand in stomach, 1.639 g per thousand in intestine and 1.590 g per thousand in kidney; the results have proved a large quantity of ingested methanol. They have also highlighted the absorption and distribution pattern of the methanol within the body.

Language: ro