Citation

Duverneuil C, de la Grandmaison GL, de Mazancourt P, Alvarez JC. Forensic Sci. Int. 2004; 141(1): 17-21.

Affiliation

Laboratoire de Pharmacologie-Toxicologie, Centre Hospitalier Universitaire Raymond Poincaré, 104 Boulevard R. Poincaré, 92380 Garches, France.

Copyright

(Copyright © 2004, Elsevier Publishing)

DOI

10.1016/j.forsciint.2003.12.010

PMID

15066709

Abstract

An original liquid chromatography method with photodiode-array detection (DAD) is presented for the determination of strychnine in blood. This sensitive method allows the use of only 0.1 ml of sample. The strychnine was isolated from blood using a liquid-liquid extraction procedure and chloroquine as an internal standard. The limits of detection (LOD) and quantification were 0.06 and 0.5 mg/l, respectively. The recovery was 94% and the coefficients of variation (CV) ranged from 5.9 to 10.8%. A fatal case of strychnine poisoning is presented, with a lethal blood concentration of 25 mg/l.

Language: en