Citation

Oehmichen M, Grüninger H, Norpoth T. Z. Rechtsmed. 1986; 97(2): 117-131.

Vernacular Title

Intravitale, azelluläre Hämolyse extravasaler Erythrozyten. Ein experimenteller Beitrag zur Wundaltersbestimmung.

Copyright

(Copyright © 1986, Holtzbrinck Springer Nature Publishing Group)

DOI

unavailable

PMID

3811629

Abstract

The time-dependent acellular, destructive changes in erythrocytes was investigated in rabbits by subcutaneous, intraperitoneal, and subdural implantation of blood-filled diffusion chambers with 0.45 micron pores. The animals were killed at various intervals after implantation (7-24 h), and the erythrocytes in the capsules were examined by light and electron microscopy. The findings were quantified by counting 100 cells per preparation under the light microscope; changes in both matrix and membrane structure were recorded. Matrix changes and membrane variants were specified by electron microscopy. Time-dependent changes in erythrocyte matrix and membrane were established, which occurred at the various localizations in an identical sequence, but with different time intervals. The destructive changes were: an increase in loss of matrix, increase in membrane permeability, and decrease in membrane stability. The physiologic bases of and the conclusions for wound timing were discussed.

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