Citation

Feng L, Xu C, Zeng X, Zhang H, Yang F, Li W, Tu Z, Li C, Hu L. Croat. Med. J. 2014; 55(5): 537-541.

Affiliation

Lan Hu, Institute of Forensic Science, Ministry of Public Security, Beijing, 100038, China, 13911162826@139.com.

Copyright

(Copyright © 2014, University of Zagreb Medical School, Publisher Medicinska Naklada)

DOI

unavailable

PMID

25358887

Abstract

Aim. To obtain individual Y-short tandem repeat (STR) profiles in a multi-suspect sexual assault case.

METHODS. We used laser cut microdissection to capture the single sperm cell in the multi-contributor semen sample, combined with the low volume polymerase chain reaction (LV-PCR) method to genotype the single sperm cell profiles using the Yfiler® kit. Consensus DNA profiles were generated from 5 replicate experiments.

RESULTS. Ninety-four parallel LV-PCRs were performed and 41 reactions (44%) produced Y-STR profiles with more than nine loci. Three individual Y-STR profiles were successfully obtained.

CONCLUSION. The three Y haplotype units matched three known perpetrators' genotypes. Our results showed that single sperm cells Y-STR analysis was a powerful method for analyzing multi-donor semen mixture sample.

Language: en