Citation

Rieck W, Platt D. J. Chromatogr. 1988; 425(1): 121-134.

Affiliation

Institut für Gerontologie, Universität Erlangen-Nürnberg, F.R.G.

Copyright

(Copyright © 1988, Elsevier Publishing)

DOI

unavailable

PMID

3360863

Abstract

A reversed-phase high-performance liquid chromatographic assay has been developed for the simultaneous determination of alpha-amanitin and phalloidin in human plasma. The procedure is based on the enrichment of the toxins on a pre-column, followed by the transfer of both compounds in a foreflush mode to the analytical column. alpha-Amanitin and phalloidin can be quantified reliably down to a minimum concentration of 10 ng/ml in plasma (relative standard deviation less than 10%). An alternative method is recommended for hepatic coma patients.

Language: en