Citation

de Paula C, Jurisch M, Piccin E, Augusti R. Drug Test. Anal. 2018; 10(9): 1348-1357.

Affiliation

Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais,, Brazil.

Copyright

(Copyright © 2018, John Wiley and Sons)

DOI

10.1002/dta.2395

PMID

29663724

Abstract

A novel methodology using liquid-liquid extraction with low temperature partitioning (LLE-LTP) and paper spray mass spectrometry (PS-MS) was developed to identify and quantify benzodiazepines in beverages. Four types of alcoholic beverages usually consumed in parties and bars were spiked with five distinct benzodiazepines (diazepam, alprazolam, bromazepam, clonazepam and cloxazolam) simulating a drug facilitated crime occurrence. The direct PS-MS analysis of the spiked beverages revealed a remarkable matrix effect with an unclear detection of protonated benzodiazepines. However, by the application of the LLE-LTP using liquid nitrogen, a prompt and doubtless detection of such compounds was achieved. The quantification potential of the LLE-LTP/ PS-MS methodology was demonstrated by using beer as matrix, diazepam as target analyte and cloxazolam as an internal standard. Figures of merit (linearity, limit of detection, linear dynamic range,relative standard deviation and recovery) were determined and adequate values were obtained. In conclusion, we demonstrated herein that the LLE-LTP/ PS-MS methodology has potential to be applied directly at the crime scene through of a portable mass spectrometer and a thermal container for the transport of liquid nitrogen.

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Language: en