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Citation |
Bouley J, Chung DY, Ayata CY, Brown RH, Henninger N. J. Neurotrauma 2019; 36(7): 1008-1017. |
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Affiliation |
University of Massachusetts Medical School, 12262, Department of Neurology , 55 Lake Ave, North , Worcester, Massachusetts, United States , 01655-0112 ; nils.henninger@umassmed.edu. |
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Copyright |
(Copyright © 2019, Mary Ann Liebert Publishers) |
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DOI |
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PMID |
29999455 |
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Abstract |
Cortical spreading depression (CSD) has been described after moderate-to-severe traumatic brain injury (TBI). However, it is uncertain whether CSD occurs after mild, concussive TBI and whether it relates to brain pathology and functional outcome. Male C57BL6/J mice (n=62) were subjected to closed head TBI with a 25g weight (n=11), 50g weight (n=45), or sham injury (n=6). Laser Doppler flowmetry and optical intrinsic signal imaging were used to determine cerebral blood flow dynamics after concussive CSD. Functional deficits were assessed at baseline, 2 h, 24 h, and 48 h. TUNEL and Prussian blue staining were used to determine cell death and presence of cerebral microbleeds at 48 h. No mouse subjected to a 25g weight drop and 58.9% of mice subjected to 50g weight drop developed a CSD. Mice with concussive CSD displayed significantly greater numbers of apoptotic cell profiles in the ipsilesional hemisphere as compared to mice without a CSD that were subjected to the same 50 g weight drop paradigm (p<0.05, each). All investigated animals had at least one cerebral microbleed (range 1 to 24). Compared to mice without a CSD, mice with a CSD had significantly more microbleeds in the traumatized hemisphere (p<0.05, each) and showed impaired recovery (p<0.05). Incidence of CSD after mild TBI depended on impact severity and was associated with histological and behavioral outcomes. These observations indicate that concussive CSD may serve as viable marker for concussion severity and provide novel avenues for outcome prediction and therapeutic decision making. Language: en |
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Keywords |
ANIMAL STUDIES; HEAD TRAUMA; IN VIVO STUDIES; TRAUMATIC BRAIN INJURY |