Citation

Wu H, Luo H, Huang F, Zhou X, Huang X, Chen J. Anal. Biochem. 2019; 569: 10-15.

Affiliation

Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, Guangdong Provincial Public Laboratory of Analysis and Testing Technology, Guangdong Institute of Analysis, Guangzhou, 510070, Guangdong, China.

Copyright

(Copyright © 2019, Elsevier Publishing)

DOI

10.1016/j.ab.2019.01.001

PMID

30639284

Abstract

A novel method for non-target screening of toxicants in poisonous honey was established in this study. Poisonous honey and nontoxic honey were contrastive detected using liquid chromatography quadrupole-time-of-flight mass spectrometry and analyzed by Mass Profiler Professional Software. 4 poisonous alkaloids were screened out and confirmed by comparison with reference compounds. In order to investigate the source of these poisonous alkaloids, 6 poisonous alkaloids, ubiquitous in Gelsemium elegan, from honey, honeybees, pollen in honeycomb and different organs of Gelsemium elegan, were quantified by liquid chromatography triple-quadrupole tandem mass spectrometry. The results showed that alkaloids composition characteristics in honey, honeybees, and pollen were similar to those in the flower and bud of Gelsemium elegan and significant different from those in leave, stem and root. This result demonstrated that poisonous alkaloids in honey were come from the gathering honey process. This strategy provided an efficient and rapid method for non-target screening of toxicants in food.

Copyright © 2019 Elsevier Inc. All rights reserved.

Language: en

Keywords

Alkaloids; Gelsemium elegan; HPLC-Q-TOF/MS; HPLC-QQQ-MS; Honey poisoning