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Citation |
Bazhenova EY, Fursenko DV, Kulikova EA, Khotskin NV, Sinyakova NA, Kulikov AA. Neurosci. Lett. 2019; 699: 91-96. |
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Affiliation |
Department of Genetic Models of Neurological Disorders, Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Science, Novosibirsk, 630090, Russia. |
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Copyright |
(Copyright © 2019, Elsevier Publishing) |
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DOI |
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PMID |
30685409 |
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Abstract |
Reduction of natural illumination in fall/winter months causes seasonal affective disorders (SAD) in vulnerable individuals. Neurotransmitter serotonin (5-HT) is involved in the mechanism of SAD. Tryptophan hydroxylase-2 (TPH2) is the key enzyme of 5-HT synthesis in the brain. C1473G polymorphism in the Tph2 gene is a key factor defining the enzyme activity in the mouse brain. The main aims of the study were to investigate the effects of C1473G polymorphism on behavior and brain 5-HT system responses to photoperiod alterations. The experiment was carried out on adult mouse males of B6-1473C and B6-1473G congenic lines with normal and low TPH2 activities, respectively. B6-1473C and B6-1473G mice were divided into four groups of 8 each and exposed for 28 days to standard-day (14h light and 10h darkness) or short-day (4h light and 20h darkness) conditions. No effect of photoperiod on locomotor, exploratory activities and anxiety in the open field test was observed. At the same time, photoperiod alterations affected depressive-like immobility in the forced swim test, the 5-HT, 5-hydroxyindoleacetic acid (5-HIAA) levels, 5-HIAA/5-HT ratio and the Htr2a mRNA level in hippocampus and midbrain. The effect of the interaction between C1473G polymorphism and photoperiod on 5-HT level and 5-HIAA/5-HT ratio in hippocampus was revealed. Short-day conditions reduced the level and increased 5-HIAA/5-HT ratio in this structure only in B6-1473G mice. At the same time, C1473G polymorphism does not alter effects of short-day conditions on immobility time in the forced swim test and the Htr2a mRNA level in the brain. Language: en |
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Keywords |
5-HT system; Behavior; C1473G polymorphism; Mice; Photoperiod; Tryptophan hydroxylase 2 |