Citation

Thaweethee-Sukjai B, Suttajit S, Thanoi S, Dalton CF, Reynolds GP, Nudmamud-Thanoi S. Int. J. Med. Sci. 2019; 16(9): 1207-1214.

Affiliation

Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand.

Copyright

(Copyright © 2019, Ivyspring International Publisher)

DOI

10.7150/ijms.36131

PMID

31588185

PMCID

PMC6775273

Abstract

Aims: To determine the extent of DNA methylation of parvalbumin gene (PVALB) promoter in major depressive disorder (MDD) patients with and without suicide attempt in comparison with healthy controls. Methods: The extracted DNA from dried blood spots of MDD patients (n = 92) including non-suicidal MDD and suicidal-MDD subgroups (n = 45 and n = 47, respectively) and age-matched control subjects (n = 95) was used for DNA methylation analysis at four CpG sites in the promoter sequence of PVALB by pyrosequencing. Results: The PVALB methylation was significantly increased at CpG2 and decreased at CpG4 in the MDD group compared to the control group, while there was no difference between non-suicidal MDD and suicidal-MDD subgroups. A significant inverse correlation of severity of MDD was indicated only for CpG4. Conclusion: This study provides the first evidence of abnormalities of PVALB promoter methylation in MDD and its correlation with MDD severity indicating a role for epigenetics in this psychiatric disorder.

© The author(s).

Language: en

Keywords

DNA methylation; major depressive disorder; parvalbumin; pyrosequencing; suicide attempt