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Citation
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Mendoza AE, Paul SR, El Hechi M, Naar L, Nederpelt C, Mikdad S, van Erp I, Hess J, Velmahos GC, Poznansky M, Reeves P. J. Trauma Acute Care Surg. 2021; ePub(ePub): ePub. |
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Abstract
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BACKGROUND: Major injury results in an early cascade of immunologic responses that increase susceptibility to infection and multiorgan dysfunction. Detailed immune profiling by mass cytometry has the potential to identify immune signatures that correspond to patient outcomes. Our objective was to determine the prognostic value of immune signatures early after major trauma injury.
METHODS: Trauma patients (n=17) were prospectively enrolled between September 2018 and December 2019. Serial whole blood samples were obtained from trauma patients (mean ISS 26.2, standard error of the mean 3.7) at days 1 and 3 after injury, and from age- and sex-matched uninjured controls using a standardized protocol for fixation, storage, and labeling. Computational analyses including K-nearest neighbor automated clustering of immune cells and Spearman's correlation analysis were used to identify correlations between cell populations, clinical measures, and patient outcomes.
RESULTS: Analysis revealed 9 immune cell clusters that correlated with one or more clinical outcomes. On days 1 & 3 post injury, the abundance of immature neutrophil and classical monocytes (cMCs) exhibited a strong positive correlation with increased ICU and hospital length of stay (LOS). Conversely, the abundance of CD4 T-cell subsets, namely Th17 cells, are associated with improved patient outcomes including: decreased ventilator-days (r =-0.76), hospital-acquired pneumonia (r=-0.69), and acute kidney injury (r=-0.73).
CONCLUSIONS: Here we provide a comprehensive multi-time point immunophenotyping analysis of whole blood from patients soon after traumatic injury to determine immune correlates of adverse outcomes. Our findings indicate that alterations in myeloid-origin cell types may contribute to immune dysfunction after injury. Conversely, presence of effector-T cell populations correspond with decreased hospital LOS and organ dysfunction. Overall, these data identify novel immune signatures following traumatic injury that support the view that monitoring of immune (sub)-populations may provide clinical decision-making support for at-risk patients early in their hospital course. LEVEL OF EVIDENCE: Basic Science paper.
Language: en |