Citation

Reyes-Garces N, Myers C. J. Sep. Sci. 2021; ePub(ePub): ePub.

Copyright

(Copyright © 2021, John Wiley and Sons)

DOI

10.1002/jssc.202001265

PMID

unavailable

Abstract

Cannabis legalization has led to the development of a variety of cannabis infused products with edibles being one of the most popular. The state of California has implemented comprehensive cannabis testing regulations requiring the analysis of cannabinoids (potency) and contaminants such as pesticides and mycotoxins in any type of cannabis good. In this work, we propose an analytical workflow for the quantification of the California list of pesticides and mycotoxins, as well as six cannabinoids, in chocolate, using 3 mL of solvent for the extraction. For the analysis of pesticides and mycotoxins, clean-up steps employing a C18 solid-phase extraction cartridge and dispersive solid-phase extraction sorbents were implemented. Gas chromatography amenable pesticides were analyzed using low-pressure gas chromatography coupled to tandem mass spectrometry which allowed for a total method run of 12 min. Both liquid chromatography and gas chromatography instrumental methods had the same analysis time, ensuring satisfactory sample throughput. For the determination of cannabinoids, a dilution of the original organic extract collected for pesticides and mycotoxins analysis (and prior to any clean-up step) was used. Excellent results in terms of linearity, accuracy, and precision were obtained for all target analytes. This article is protected by copyright. All rights reserved.

Language: en

Keywords

cannabis; cannabinoids; California pesticides and mycotoxins; chocolate; low-pressure gas chromatography