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Journal Article

Citation

Hariri RJ, Chang VA, Barie PS, Wang RS, Sharif SF, Ghajar JB. Brain Res. 1994; 636(1): 139-142.

Affiliation

Aitken Neurosurgery Laboratory, Department of Surgery, Cornell University Medical College, New York, NY 10021.

Copyright

(Copyright © 1994, International Brain Research Organization, Publisher Elsevier Publishing)

DOI

unavailable

PMID

8156401

Abstract

The brain is being evaluated as a de novo source of cytokines. Because recent evidence indicates that interleukin-6 (IL-6) may influence blood-brain barrier function and vascular permeability, we have sought to determine whether mechanical injury can directly induce in situ cerebral IL-6 production. Adult human astrocyte cultures were subjected to mechanical injury by the in vitro method of fluid percussion barotrauma, developed in our laboratory. Serial supernatant samples were collected for 8 h and evaluated for IL-6 activity using a proliferation assay employing the dependent B cell hybridoma cell line, B9. At optimum injury, the IL-6 level became significantly (P < 0.0001, analysis of variance) elevated from baseline 2 h after trauma and continued to increase over the observation period. Our study shows that following mechanical injury human astrocytes produce IL-6, which may contribute to post-traumatic cerebrovascular dysfunction. Elucidating the precise role of intracerebral cytokines is essential to our understanding of the mechanism responsible for post-traumatic cerebrovascular dysfunction.


Language: en

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