Citation

Ottens AK, Bustamante L, Golden EC, Yao C, Hayes RL, Wang KK, Tortella FC, Dave JR. J. Neurotrauma 2010; 27(10): 1837-1852.

Affiliation

Virginia Commonwealth University, Anatomy & Neurobiology and Biochemistry, Richmond, Virginia, United States; akottens@vcu.edu.

Copyright

(Copyright © 2010, Mary Ann Liebert Publishers)

DOI

10.1089/neu.2010.1374

PMID

20698760

PMCID

PMC2953930

Abstract

Diagnosis and treatment of stroke and traumatic brain injury remain significant healthcare challenges to society. Patient care stands to benefit from an improved understanding of the interactive biochemistry underlying neurotrauma pathobiology. In this study, we assessed the power of neuroproteomics to contrast biochemical responses following ischemic and traumatic brain injuries in the rat. A middle cerebral artery occlusion (MCAo) model was employed in groups of 30-min and 2-h focal neocortical ischemia with reperfusion. Neuroproteomes were assessed via cationic anionic exchange chromatography - gel electrophoresis followed by reversed-phase liquid chromatography - tandem mass spectrometry. MCAo results were compared with those from a previous study of focal contusional brain injury employing the same methodology to characterize homologous neocortical tissues at two days post injury. The 30-min MCAo neuroproteome depicted abridged energy production involving pentose phosphate, modulated synaptic function and plasticity, and increased chaperone activity and cell survival factors. The 2-h MCAo data denoted near complete loss of ATP production, synaptic dysfunction with degraded cytoarchitecture, more conservative chaperone activity and additional cell survival factors than in the 30-min MCAo. The TBI group exhibited disrupted metabolism, but with retained malate shuttle functionality. Synaptic dysfunction and cytoarchitectural degradation resembled the 2-h MCAo group; however, chaperone and cell survival factors were more depressed following TBI. These results underscore the utility of neuroproteomics at characterizing interactive biochemistry for profiling and contrasting molecular aspects underlying pathobiological differences between brain injuries.

Language: en